UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BELIMBING WULUH (Averrhoa bilimbi, L.) TERHADAP 1,1-DIPHENYL-2-PICRYLHIDRAZYL (DPPH)

Ilham Kuncahyo, Sunardi
D-III Teknologi Farmasi Fakultas Teknik Universitas Setia Budi
e-mail : nardi_usb@yahoo.com

ABSTRACT
Sour carambola (Averrhoa bilimbi, L.) constitute one of plants than can be used as an
antioxidant. This research aims to find out the potential antioxidant activity of ether fraction and
methanolic extract liquid of sour carambola leaves on the free radical DPPH. The Sour carambola
leaves powder was extracted using soxhlet tool with petroleum ether solvent followed with
methanol solvent. The methanolic extract obtained was suspended with water and partitioned with
ether. The ether and aqueous fraction were made in various concentrations: 10, 20, 40, 80 and 160
ppm. Then DPPH solution was added to it. Absorbance reading was conducted with
spectrophotometry at the wave length of 517 nm after 30 minutes. The control used was routine.
The result of research shows the potential as antioxidant scavenging free radical with ether
fraction value IC50 of 50.36 ppm and liquid fraction of 44.01 ppm. With routine as the control it is
obtained IC50 value of 7.00 ppm. The one-way Anava statistic test was conducted on the IC50 values
of those three tested solution to find out the significant differences.
Keywords: Antioxidant, sour carambolas, ether fraction, aqueous, DPPH.