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Sri Komarayati, Ina Winarni & Djarwanto1
1 Pusat Penelitian dan Pengembangan Keteknikan Kehutanan dan Pengolahan Hasil Hutan,Jl. Gunung Batu No. 5, Bogor 16610, Telp. 0251- 8633378, Fax. 0251-8633413
1 Pusat Penelitian dan Pengembangan Keteknikan Kehutanan dan Pengolahan Hasil Hutan,Jl. Gunung Batu No. 5, Bogor 16610, Telp. 0251- 8633378, Fax. 0251-8633413
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ABSTRACT
Sago , an endemic plant of Indonesia, is potential for bio-ethanol basematerial. Bioethanol can be producted from sago core, starch as well as fiber by utilizing certainenzymes. This research was conducted to determine the effectivenees of alpha amylase and gluco-amylase enzymes for bio-ethanol production. The process employed in this research include delignification using alpha amylase enzyme, saccharification using gluco amylase enzyme, and 3-day fermentation at temperature 28 C the process concluded with distillation at 78-100 C.
Laboratory results showed that producing ethanol from sago strach with a combination of 0.18mL/liter alpha amylase and 0.48 ml/liter gluco amylase gives the highest percentage of bio-ethanol, namely 65%. The combination of 0.13 mL/liter alpha amylase, 0.11 mL/liter gluco amylase and 0.33g/liter yeast on sago core only produces 44.84% ethanol using the same combination on sago fiber gives as low as 8.26% ethanol. At the small scale, production using strach, core and fiber gives 24%, 11% and 4% ethanol respectively.
Laboratory results showed that producing ethanol from sago strach with a combination of 0.18mL/liter alpha amylase and 0.48 ml/liter gluco amylase gives the highest percentage of bio-ethanol, namely 65%. The combination of 0.13 mL/liter alpha amylase, 0.11 mL/liter gluco amylase and 0.33g/liter yeast on sago core only produces 44.84% ethanol using the same combination on sago fiber gives as low as 8.26% ethanol. At the small scale, production using strach, core and fiber gives 24%, 11% and 4% ethanol respectively.
Keywords : Sago, enzyme -amilase, enzim glukoamilase, bioetanol
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